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Lacin Lab | Neural Circuit Development & Behavior

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Protocols

How to dissect the fly CNS

Larval Dissection

By Lacin Lab at UMKC

By Tim Lebestky, Williams College

Pupal Dissection

48APF, By Sam Herman, Lacin Lab

Pupal dissection From Tea et al.,

Adult Dissection

VNC dissection of glued decapitated flies, by Lacin Lab
From Janelia FlyLight
From 映像 

Immuno-based Staining

  • Embryo/Larva Protocols by N. Patel

FlyLight Protocols

  • Larval CNS fixation
  • Adult CNS fixation
  • Staining
  • DPX based mounting
  • Glycerol based mounting
  • More protocols by FlyLight
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